Hi Chris, That's very interesting about IB and ILT vaccines. I had heard they weren't a problem to mix with unvaccinated birds. I found an article about mycoplasma vaccines. The main one used is a live vaccine, and the bird then carries mycoplasma for life. Perhaps as you say it is a mild strain which will only affect weak birds. I took the two chicks I bought to a specialist poultry vet yesterday. She thinks it is possible that the one that became ill had a yolk sac infection as this causes a terrible smell, and this may have been what made it ill. It is often a yolk sac issue which causes the runting. She said many free-ranging birds pick up mycoplasma from wild birds, but that if they hadn't been exposed, they could become very ill from it. I therefore shouldn't mix them with mine if the blood tests are positive. She is testing the chicks for a whole range of things for just £20. I was relieved that the blood test wasn't too bad done by an expert with a fine needle.